产品描述

This Human SERPINA7 ELISA Kit was designed for the quantitative measurement of Human SERPINA7 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 12.5 ng/mL-200 ng/mL and the sensitivity is 12.5 ng/mL.

别名

Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 7 ELISA Kit; Serpin A7 ELISA Kit; Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 7 ELISA Kit; Serpin peptidase inhibitor, clade A, member 7 ELISA Kit; Serpina7 ELISA Kit; T4 binding globulin ELISA Kit; TBG ELISA Kit; TBG, serum ELISA Kit; THBG_HUMAN ELISA Kit; Thyroxin binding globulin ELISA Kit; Thyroxine binding globulin ELISA Kit; Thyroxine-binding globulin ELISA Kit; Thyroxine-binding globulin od serum ELISA Kit

缩写

SERPINA7

Uniprot No

P05543

灵敏度

12.5 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Others

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human TBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		91
		Range %		85-96
1:2		Average %		96
		Range %		92-105
1:4		Average %		84
		Range %		80-89
1:8		Average %		91
		Range %		85-98

回收率

The recovery of human TBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		91		87-95
EDTA plasma (n=4)		96		90-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average     200 0.121 0.115 0.118     100 0.328 0.328 0.328     50 0.513 0.312 0.413     25 1.015 0.497 0.756     12.5 2.116 2.121 2.119

货期

3-5 working days