产品描述
CUSABIO's rat podocalyxin (PCX) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat PCX in serum, urine, or cell culture supernates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of PCX in samples. The color intensity is measured at 450 nm via a microplate reader.
PCX, also called PODXL, is a type I membrane mucoprotein abundantly presented in the podocytes of kidney glomeruli where it plays an important role in maintaining the structural organization of the kidney glomeruli and normal plasma filtration. Dominant and recessive mutations in PODXL are linked to human kidney disease. Abnormal expression of PODXL has been implicated in a range of cancers. PODXL is upregulated on tumors with high metastatic index. The presence of PODXL is associated with poor outcomes, thus emerging as an important prognostic and theragnostic marker in several human cancers.
别名
Podxl ELISA Kit; Pclp1 ELISA Kit; Podocalyxin ELISA Kit; Podocalyxin-like protein 1 ELISA Kit; PC ELISA Kit; PCLP-1 ELISA Kit
缩写
PODXL
Uniprot No
Q9WTQ2
灵敏度
0.39 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PCX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 87 | |
| Range % | 81-93 | |
| 1:2 | Average % | 97 | |
| Range % | 93-101 | |
| 1:4 | Average % | 89 | |
| Range % | 85-93 | |
| 1:8 | Average % | 98 | |
| Range % | 92-104 | |
回收率
| The recovery of rat PCX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 91 | 85-97 | |
| EDTA plasma (n=4) | 89 | 83-94 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 100 | 2.201 | 2.312 | 2.257 | 2.112 | | | 50 | 1.574 | 1.456 | 1.515 | 1.370 | | | 25 | 1.009 | 1.056 | 1.033 | 0.888 | | | 12.5 | 0.633 | 0.654 | 0.644 | 0.499 | | | 6.25 | 0.401 | 0.412 | 0.407 | 0.262 | | | 3.12 | 0.272 | 0.286 | 0.279 | 0.134 | | | 1.56 | 0.210 | 0.215 | 0.213 | 0.068 | | | 0 | 0.144 | 0.146 | 0.145 | | |
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货期
3-5 working days
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