产品描述
SELE(E-selectin):SELE是一种细胞黏附分子,属于血管内皮细胞黏附分子家族,主要通过与其上游调控区的L-选择素(L-selectin)和P-选择素(P-selectin)结合,参与炎症和免疫反应中白细胞的黏附和转移。SELE的表达受多种因素的调控,如TNF-α、IL-1、LPS等。在某些疾病状态中,SELE的表达会增加,因此SELE也被认为是一种炎症指标。
华美生物所提供的Mouse E-Selectin ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测鼠血清、血浆、组织匀浆样本中的SELE,其灵敏度为19.5 pg/mL,检测范围为78 pg/mL-5000 pg/mL。
别名
Sele ELISA Kit; Elam-1 ELISA Kit; E-selectin ELISA Kit; CD62 antigen-like family member E ELISA Kit; Endothelial leukocyte adhesion molecule 1 ELISA Kit; ELAM-1 ELISA Kit; Leukocyte-endothelial cell adhesion molecule 2 ELISA Kit; LECAM2 ELISA Kit; CD antigen CD62E ELISA Kit
缩写
SELE
Uniprot No
Q00690
灵敏度
19.5 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
|
| |
|
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse E-Selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
|
| |
Sample |
Serum(n=4) |
|
|
| 1:1 |
Average % |
93 |
|
|
| Range % |
90-96 |
|
|
| 1:2 |
Average % |
85 |
|
|
| Range % |
81-90 |
|
|
| 1:4 |
Average % |
104 |
|
|
| Range % |
100-108 |
|
|
| 1:8 |
Average % |
86 |
|
|
| Range % |
82-90 |
|
|
回收率
| The recovery of mouse E-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| |
| Sample Type |
Average % Recovery |
Range |
|
|
| Serum (n=5) |
102 |
98-106 |
|
|
| EDTA plasma (n=4) |
89 |
85-94 |
|
|
| |
|
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
|
| 5000 |
2.076 |
2.131 |
2.104 |
1.907 |
|
|
| 2500 |
1.671 |
1.638 |
1.655 |
1.458 |
|
|
| 1250 |
1.168 |
1.201 |
1.185 |
0.988 |
|
|
| 625 |
0.829 |
0.831 |
0.830 |
0.633 |
|
|
| 312 |
0.505 |
0.511 |
0.508 |
0.311 |
|
|
| 156 |
0.365 |
0.372 |
0.369 |
0.172 |
|
|
| 78 |
0.285 |
0.289 |
0.287 |
0.090 |
|
|
| 0 |
0.195 |
0.199 |
0.197 |
|
|
|
|
货期
3-5 working days