产品描述

This human PPARGC1A ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human PPARGC1A in different samples, including serum, plasma, cell culture supernates, or cell lysates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of PPARGC1A bound in the initial step.

PPARGC1A is involved in biological functions with implications for insulin action including protection against oxidative stress, formation of muscle fiber types as well as regulation of microvascular flow. It is a chief regulator of energy metabolism and mitochondrial biogenesis by integrating and coordinating the activity of other transcription factors such as Nrf1, nuclear factor 2, PPARα, and mitochondrial transcription A. PPARGC1A is also central to antioxidant defense and redox balance, by regulating the expression of factors such as Nrf2 and MnSOD and promoting NADPH generation, and thereby counters increased ROS levels in highly oxidative cells and protects against inflammation. It is involved in the progression of hormone-associated cancers.

别名

L PGC 1alpha ELISA Kit; LEM6 ELISA Kit; Ligand effect modulator 6 ELISA Kit; Peroxisome proliferative activated receptor gamma coactivator 1 alpha ELISA Kit; Peroxisome proliferative activated receptor gamma coactivator 1 ELISA Kit; Peroxisome proliferator activated receptor gamma coactivator 1 alpha ELISA Kit; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 3ext ELISA Kit; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 8a ELISA Kit; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 ELISA Kit; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 ELISA Kit; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 NT ELISA Kit; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha ELISA Kit; PGC 1 (alpha) ELISA Kit; PGC 1 alpha ELISA Kit; PGC 1v ELISA Kit; PGC-1-alpha ELISA Kit; PGC1 ELISA Kit; PGC1(alpha) ELISA Kit; PGC1A ELISA Kit; PGC1v ELISA Kit; PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6 ELISA Kit; PPAR gamma coactivator 1 alpha ELISA Kit; PPAR gamma coactivator 1 ELISA Kit; PPAR gamma coactivator variant form ELISA Kit; PPAR-gamma coactivator 1-alpha ELISA Kit; PPARGC 1 alpha ELISA Kit; PPARGC-1-alpha ELISA Kit; PPARGC1 ELISA Kit; PPARGC1A ELISA Kit; PRGC1_HUMAN ELISA Kit

缩写

PPARGC1A

Uniprot No

Q9UBK2

灵敏度

31.25 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PPARGC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		84
		Range %		80-92
1:2		Average %		97
		Range %		90-105
1:4		Average %		99
		Range %		92-110
1:8		Average %		95
		Range %		86-99

回收率

The recovery of human PPARGC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-100
EDTA plasma (n=4)		96		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 8000 2.054 2.052 2.053 1.954 4000 1.315 1.325 1.320 1.221 2000 0.762 0.777 0.770 0.671 1000 0.470 0.477 0.474 0.375 500 0.284 0.295 0.290 0.191 250 0.216 0.224 0.220 0.121 125 0.157 0.165 0.161 0.062 0 0.096 0.102 0.099

货期

3-5 working days