产品描述

This Mouse PTGS2 ELISA Kit was designed for the quantitative measurement of Mouse PTGS2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

别名

Ptgs2 ELISA Kit; Cox-2 ELISA Kit; Cox2 ELISA Kit; Pghs-b ELISA Kit; Tis10 ELISA Kit; Prostaglandin G/H synthase 2 ELISA Kit; EC 1.14.99.1 ELISA Kit; Cyclooxygenase-2 ELISA Kit; COX-2 ELISA Kit; Glucocorticoid-regulated inflammatory cyclooxygenase ELISA Kit; Gripghs ELISA Kit; Macrophage activation-associated marker protein P71/73 ELISA Kit; PES-2 ELISA Kit; PHS II ELISA Kit; Prostaglandin H2 synthase 2 ELISA Kit; PGH synthase 2 ELISA Kit; PGHS-2 ELISA Kit; Prostaglandin-endoperoxide synthase 2 ELISA Kit; TIS10 protein ELISA Kit

缩写

PTGS2

Uniprot No

Q05769

灵敏度

7.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of mouse COX-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		88
		Range %		80-92
1:2		Average %		98
		Range %		91-105
1:4		Average %		100
		Range %		92-110
1:8		Average %		93
		Range %		86-98

回收率

The recovery of mouse COX-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		96		89-98
EDTA plasma (n=4)		96		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 2000 2.660 2.654 2.657 2.547 1000 2.107 2.046 1.994 1.884 500 1.494 1.385 1.440 1.330 250 0.932 0.911 0.922 0.812 125 0.502 0.489 0.496 0.386 62.5 0.403 0.398 0.415 0.305 31.25 0.223 0.207 0.215 0.105 0 0.114 0.106 0.110

货期

3-5 working days