产品描述
RETN(Retinol binding protein 4)中文名为视黄醇结合蛋白4,是一种由肝脏合成并用于运输维生素A的血浆蛋白。此外,它还可以调节脂肪酸的代谢和胰岛素敏感性,与糖尿病和肥胖相关。RETN的含量在糖尿病、代谢综合征、脂肪肝等疾病中明显升高。它还参与了许多疾病的发生和发展,如肥胖、心脑血管疾病和恶性肿瘤。
华美生物所提供的Mouse Resistin ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测鼠血清、血浆、细胞培养上清、组织匀浆样本中的RETN,其灵敏度为41.036 pg/mL,检测范围为62.5 pg/mL-4000 pg/mL。
别名
Retn ELISA Kit; Fizz3 ELISA Kit; Resistin ELISA Kit; Adipose tissue-specific secretory factor ELISA Kit; ADSF ELISA Kit; Adipose-specific cysteine-rich secreted protein A12-alpha ELISA Kit; Cysteine-rich secreted protein FIZZ3 ELISA Kit
缩写
RETN
Uniprot No
Q99P87
灵敏度
41.036 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse resistin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
96 |
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| Range % |
91-103 |
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| 1:200 |
Average % |
85 |
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| Range % |
80-90 |
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| 1:400 |
Average % |
93 |
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| Range % |
89-98 |
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| 1:800 |
Average % |
100 |
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| Range % |
94-105 |
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回收率
| The recovery of mouse resistin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
91 |
85-96 |
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| EDTA plasma (n=4) |
94 |
90-98 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 4000 |
2.524 |
2.485 |
2.505 |
2.318 |
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| 2000 |
1.917 |
2.007 |
1.962 |
1.775 |
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| 1000 |
1.294 |
1.352 |
1.323 |
1.136 |
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| 500 |
0.893 |
0.849 |
0.871 |
0.684 |
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| 250 |
0.627 |
0.601 |
0.614 |
0.427 |
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| 125 |
0.421 |
0.447 |
0.434 |
0.247 |
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| 62.5 |
0.297 |
0.285 |
0.291 |
0.104 |
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| 0 |
0.190 |
0.183 |
0.187 |
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货期
3-5 working days