产品描述
甲状腺素(T3)是一种由甲状腺合成的激素,对人体的代谢、生长发育和神经系统发育等方面起着至关重要的作用。它的分泌受到下丘脑和垂体的调控,通过影响基础代谢率、心脏和肌肉的收缩、神经元的生长和分化等,对身体产生了广泛的影响。T3的过多或不足都可能导致各种健康问题,如心血管疾病、代谢性疾病、肌无力等。
华美生物所提供的Pig Tri-iodothyronine,T3 ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测猪血清、血浆样本中的T3,其灵敏度为500 pg/mL,检测范围为500 pg/mL-8000 pg/mL。
缩写
T3
灵敏度
500 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
|
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
|
| Inter-assay Precision (Precision between assays): CV%<15% |
|
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
|
| |
|
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pigT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
|
| |
Sample |
Serum(n=4) |
|
|
| 1:1 |
Average % |
95 |
|
|
| Range % |
90-100 |
|
|
| 1:2 |
Average % |
98 |
|
|
| Range % |
87-105 |
|
|
| 1:4 |
Average % |
92 |
|
|
| Range % |
85-100 |
|
|
| 1:8 |
Average % |
95 |
|
|
| Range % |
90-102 |
|
|
回收率
| The recovery of pig T3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| |
| Sample Type |
Average % Recovery |
Range |
|
|
| Serum (n=5) |
94 |
90-98 |
|
|
| EDTA plasma (n=4) |
97 |
93-102 |
|
|
| |
|
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| |
| pg/ml |
OD1 |
OD2 |
Average |
|
|
|
| 8000 |
0.128 |
0.130 |
0.129 |
|
|
|
| 4000 |
0.237 |
0.242 |
0.240 |
|
|
|
| 2000 |
0.556 |
0.559 |
0.558 |
|
|
|
| 1000 |
1.156 |
1.125 |
1.141 |
|
|
|
| 500 |
2.481 |
2.428 |
2.455 |
|
|
|
|
货期
3-5 working days