产品描述
T3是一种甲状腺激素,对于机体的代谢和发育有重要影响。它通过结合甲状腺激素受体,影响基因转录和蛋白质合成。T3水平的调节对于机体内平衡的维持至关重要,过低或过高的T3水平都会对身体产生负面影响。因此,T3在甲状腺功能异常、代谢异常等疾病的诊断和治疗中具有重要作用。
华美生物所提供的Goat Tri-iodothyronine(T3) ELISA KIT属于ELISA检测试剂盒,采用竞争法定量检测山羊血清、血浆、组织匀浆样本中的T3,其灵敏度为0.5 ng/mL,检测范围为0.5 ng/mL-8 ng/mL。
缩写
T3
灵敏度
0.5 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of goat T3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
90-103 |
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| 1:2 |
Average % |
95 |
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| Range % |
85-102 |
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| 1:4 |
Average % |
93 |
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| Range % |
90-97 |
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| 1:8 |
Average % |
94 |
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| Range % |
88-99 |
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回收率
| The recovery of goat T3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
86-102 |
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| EDTA plasma (n=4) |
96 |
92-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
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| 8 |
0.166 |
0.168 |
0.167 |
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| 4 |
0.281 |
0.288 |
0.285 |
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| 2 |
0.558 |
0.553 |
0.556 |
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| 1 |
1.262 |
1.247 |
1.255 |
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| 0.5 |
2.105 |
2.138 |
2.122 |
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货期
3-5 working days