产品描述
This Human Pro-ADM ELISA Kit was designed for the quantitative measurement of Human Pro-ADM protein in serum, plasma, cell culture supernates, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 pmol/L-20 pmol/L and the sensitivity is 0.078 pmol/L.
缩写
Pro-ADM
灵敏度
0.078 pmol/L
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human Pro-ADM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:200 | Average % | 85 | |
| Range % | 80-92 | |
| 1:400 | Average % | 95 | |
| Range % | 90-100 | |
| 1:800 | Average % | 99 | |
| Range % | 92-110 | |
| 1:1600 | Average % | 92 | |
| Range % | 82-105 | |
回收率
| The recovery of human Pro-ADM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 93 | 89-100 | |
| EDTA plasma (n=4) | 101 | 90-110 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pmol/L | OD1 | OD2 | Average | Corrected | | | 20 | 3.052 | 2.952 | 3.002 | 2.860 | | | 10 | 2.587 | 2.476 | 2.532 | 2.390 | | | 5 | 1.819 | 1.704 | 1.762 | 1.620 | | | 2.5 | 1.125 | 1.109 | 1.117 | 0.975 | | | 1.25 | 0.702 | 0.684 | 0.693 | 0.551 | | | 0.625 | 0.406 | 0.389 | 0.398 | 0.256 | | | 0.312 | 0.273 | 0.258 | 0.266 | 0.124 | | | 0 | 0.147 | 0.137 | 0.142 | | |
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货期
3-5 working days