产品描述

This Human PPIA ELISA Kit was designed for the quantitative measurement of Human PPIA protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.

别名

Cyclophilin A ELISA Kit; Cyclophilin ELISA Kit; CyclophilinA ELISA Kit; Cyclosporin A binding protein ELISA Kit; Cyclosporin A-binding protein ELISA Kit; CYPA ELISA Kit; CYPH ELISA Kit; Epididymis secretory sperm binding protein Li 69p ELISA Kit; HEL S 69p ELISA Kit; MGC117158 ELISA Kit; MGC12404 ELISA Kit; MGC23397 ELISA Kit; Peptidyl prolyl cis trans isomerase A ELISA Kit; Peptidyl-prolyl cis-trans isomerase A ELISA Kit; Peptidylprolyl isomerase A (cyclophilin A) ELISA Kit; Peptidylprolyl isomerase A ELISA Kit; PPIA ELISA Kit; PPIA protein ELISA Kit; PPIA_HUMAN ELISA Kit; PPIase A ELISA Kit; Rotamase A ELISA Kit; RotamaseA ELISA Kit; T cell cyclophilin ELISA Kit

缩写

PPIA

Uniprot No

P62937

灵敏度

0.78 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PPIA/CYPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		87-95
1:2		Average %		100
		Range %		95-104
1:4		Average %		90
		Range %		85-94
1:8		Average %		98
		Range %		92-101

回收率

The recovery of human PPIA/CYPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		84-94
EDTA plasma (n=4)		95		89-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 200 2.079 2.035 2.057 1.948 100 1.594 1.576 1.585 1.476 50 0.849 0.831 0.840 0.731 25 0.446 0.439 0.443 0.334 12.5 0.299 0.284 0.292 0.183 6.25 0.204 0.198 0.201 0.092 3.12 0.153 0.158 0.156 0.047 0 0.110 0.108 0.109

货期

3-5 working days