产品描述
POSTN(Periostin)是一种细胞外基质蛋白质,参与了骨骼、心脏和肺部的发育和修复过程。此外,它还在某些癌症的发展中起到了作用。近年来的研究表明,POSTN还可能在哮喘和过敏性疾病中扮演重要角色。人体内POSTN的含量较低,但在某些疾病中,如骨质增生等,POSTN的含量会增加。
华美生物所提供的Mouse periostin/osteoblast specific factor 2 (POSTN) ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测鼠 血清、血浆、组织匀浆样本中的POSTN,其灵敏度为0.039 ng/mL,检测范围为1.56 ng/mL-100 ng/mL。
别名
Postn ELISA Kit; Osf2 ELISA Kit; Periostin ELISA Kit; PN ELISA Kit; Osteoblast-specific factor 2 ELISA Kit; OSF-2 ELISA Kit
缩写
POSTN
Uniprot No
Q62009
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse POSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1000 |
Average % |
103 |
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| Range % |
94-107 |
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| 1:2000 |
Average % |
96 |
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| Range % |
85-100 |
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| 1:4000 |
Average % |
91 |
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| Range % |
85-95 |
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| 1:8000 |
Average % |
91 |
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| Range % |
82-94 |
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回收率
| The recovery of mouse POSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
84-96 |
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| EDTA plasma (n=4) |
90 |
83-95 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.511 |
2.435 |
2.473 |
2.299 |
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| 5 |
2.101 |
2.075 |
2.088 |
1.914 |
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| 2.5 |
1.452 |
1.499 |
1.476 |
1.302 |
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| 1.25 |
0.925 |
0.938 |
0.932 |
0.758 |
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| 0.625 |
0.549 |
0.573 |
0.561 |
0.387 |
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| 0.312 |
0.402 |
0.414 |
0.408 |
0.234 |
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| 0.156 |
0.284 |
0.293 |
0.289 |
0.115 |
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| 0 |
0.172 |
0.176 |
0.174 |
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货期
3-5 working days