产品描述
The human Trypsin ELISA kit is suitable for the quantitative determination of human Trypsin in different sample types, including serum and plasma. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify antigen levels in the sample. The amount of synthesized colored products is positively related to the analyte of interest in the sample.
Trypsin (PRSS1) is a serine protease produced in the pancreas in the precursor form of inactive trypsinogen by enterokinase. Trypsin is renowned for its role in enteric digestion. In the small intestine, trypsin breaks down proteins, continuing the process of digestion that began in the stomach. It has potent proteolytic activity, and inappropriate activation of trypsin may result in severe pathological states in various human diseases. Extra-pancreatic expression of trypsin has been detected in several cancer types. Trypsin is involved in colorectal carcinogenesis and promotes proliferation, invasion, and metastasis.
别名
Alpha-trypsin chain 2 ELISA Kit; Beta-trypsin ELISA Kit; Cationic trypsinogen ELISA Kit; Digestive zymogen ELISA Kit; Nonfunctional trypsin 1 ELISA Kit; Prss1 ELISA Kit; Serine protease 1 ELISA Kit; TCR V beta 4.1 ELISA Kit; TRP1 ELISA Kit; TRY1 ELISA Kit; TRY1_HUMAN ELISA Kit; TRY4 ELISA Kit; TRYP1 ELISA Kit; Trypsin I ELISA Kit; Trypsinogen 1 ELISA Kit; Trypsinogen A ELISA Kit
缩写
PRSS1
Uniprot No
P07477
灵敏度
15.6 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
|
| 1:1000 |
Average % |
95 |
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| Range % |
91-102 |
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| 1:2000 |
Average % |
93 |
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| Range % |
88-99 |
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| 1:4000 |
Average % |
89 |
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| Range % |
82-97 |
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| 1:8000 |
Average % |
96 |
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| Range % |
91-108 |
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回收率
| The recovery of human trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
89-99 |
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| EDTA plasma (n=4) |
96 |
90-104 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 4000 |
2.901 |
2.884 |
2.893 |
2.759 |
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| 2000 |
2.491 |
2.481 |
2.486 |
2.352 |
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| 1000 |
1.736 |
1.756 |
1.746 |
1.612 |
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| 500 |
0.942 |
0.955 |
0.949 |
0.815 |
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| 250 |
0.507 |
0.511 |
0.509 |
0.375 |
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| 125 |
0.462 |
0.473 |
0.468 |
0.334 |
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| 62.5 |
0.258 |
0.252 |
0.255 |
0.121 |
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| 0 |
0.132 |
0.136 |
0.134 |
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货期
3-5 working days
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