产品描述
纤溶酶原(PLG)是一种酶原性蛋白,它能够转化为纤溶酶,参与机体的纤溶系统。在某些疾病状态下,PLG的水平可能会显著升高或降低,成为评估这些疾病的生物标志物之一。
华美生物所提供的Mouse Plasminogen(PLG) ELISA kit属于ELISA检测试剂盒,采用竞争法定量检测鼠血清、血浆、组织匀浆样本中的PLG,其灵敏度为15.63 ng/mL,检测范围为62.5 ng/mL-4000 ng/mL。
别名
Plg ELISA Kit; Plasminogen ELISA Kit; EC 3.4.21.7) [Cleaved into: Plasmin heavy chain A; Activation peptide; Angiostatin; Plasmin heavy chain A ELISA Kit; short form; Plasmin light chain B] ELISA Kit
缩写
PLG
Uniprot No
P20918
灵敏度
15.63 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PLG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
98 |
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| Range % |
92-103 |
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| 1:200 |
Average % |
97 |
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| Range % |
93-102 |
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| 1:400 |
Average % |
104 |
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| Range % |
98-108 |
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| 1:800 |
Average % |
86 |
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| Range % |
81-92 |
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回收率
| The recovery of mouse PLG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
87 |
83-93 |
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| EDTA plasma (n=4) |
94 |
88-98 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
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| 4000 |
0.098 |
0.099 |
0.099 |
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| 2000 |
0.125 |
0.132 |
0.129 |
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| 1000 |
0.179 |
0.184 |
0.182 |
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| 500 |
0.290 |
0.308 |
0.299 |
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| 250 |
0.462 |
0.475 |
0.469 |
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| 125 |
0.758 |
0.788 |
0.773 |
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| 62.5 |
1.015 |
1.103 |
1.059 |
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| 0 |
2.002 |
2.110 |
2.056 |
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货期
3-5 working days