产品描述

This Human SIRT3 ELISA Kit was designed for the quantitative measurement of Human SIRT3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .

别名

hSIRT 3 ELISA Kit; hSIRT3 ELISA Kit; Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase ELISA Kit; NAD dependent deacetylase sirtuin 3 mitochondrial ELISA Kit; NAD-dependent protein deacetylase sirtuin-3, mitochondrial ELISA Kit; Regulatory protein SIR2 homolog 3 ELISA Kit; Silent mating type information regulation 2 S.cerevisiae homolog 3 ELISA Kit; Sir 2 like 3 ELISA Kit; SIR 2 like protein 3 ELISA Kit; SIR 3 ELISA Kit; SIR2 L3 ELISA Kit; Sir2 like 3 ELISA Kit; SIR2 like protein 3 ELISA Kit; SIR2-like protein 3 ELISA Kit; SIR2L3 ELISA Kit; SIR3_HUMAN ELISA Kit; SIRT 3 ELISA Kit; SIRT3 ELISA Kit; Sirtuin 3 ELISA Kit; Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae) ELISA Kit; Sirtuin type 3 ELISA Kit; Sirtuin3 ELISA Kit

缩写

SIRT3

Uniprot No

Q9NTG7

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human SIRT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		89
		Range %		82-95
1:2		Average %		92
		Range %		86-99
1:4		Average %		98
		Range %		90-105
1:8		Average %		93
		Range %		88-100

回收率

The recovery of human SIRT3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		90-103
EDTA plasma (n=4)		90		84-97

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 2.743 2.612 2.678 2.540 500 2.312 2.156 2.234 2.096 250 1.713 1.686 1.700 1.562 125 1.021 1.009 1.015 0.877 62.5 0.514 0.557 0.536 0.398 31.2 0.321 0.301 0.311 0.173 15.6 0.203 0.196 0.200 0.062 0 0.139 0.137 0.138

货期

3-5 working days