产品描述

    PTGS2 (环氧合酶-2, prostaglandin-endoperoxide synthase 2) 是一种酶类蛋白，是合成前列腺素E2的关键酶之一。PTGS2广泛分布于人体各个组织和细胞中，其合成的前列腺素E2在多种生理和病理过程中发挥着重要作用，如炎症反应、免疫反应、细胞增殖和凋亡、肿瘤发生和发展等。在某些疾病如风湿性关节炎和肿瘤中，PTGS2的表达水平明显升高。

别名

Ptgs2 ELISA Kit; Cox-2 ELISA Kit; Cox2 ELISA Kit; Prostaglandin G/H synthase 2 ELISA Kit; EC 1.14.99.1 ELISA Kit; Cyclooxygenase-2 ELISA Kit; COX-2 ELISA Kit; PHS II ELISA Kit; Prostaglandin H2 synthase 2 ELISA Kit; PGH synthase 2 ELISA Kit; PGHS-2 ELISA Kit; Prostaglandin-endoperoxide synthase 2 ELISA Kit

缩写

PTGS2

Uniprot No

P35355

灵敏度

0.39 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of rat COX-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		98
		Range %		92-106
1:2		Average %		95
		Range %		91-99
1:4		Average %		92
		Range %		88-96
1:8		Average %		85
		Range %		80-89

回收率

The recovery of rat COX-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		88-98
EDTA plasma (n=4)		94		90-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected   100 2.386 2.285 2.336 2.191   50 1.628 1.527 1.578 1.433   25 0.937 0.916 0.927 0.782   12.5 0.564 0.575 0.570 0.425   6.25 0.365 0.376 0.371 0.226   3.12 0.240 0.251 0.246 0.101   1.56 0.195 0.191 0.193 0.048   0 0.145 0.144 0.145

货期

3-5 working days