产品描述

This Human SERPINF2 ELISA Kit was designed for the quantitative measurement of Human SERPINF2 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .

别名

A2AP ELISA Kit; A2AP_HUMAN ELISA Kit; AAP ELISA Kit; Alpha 2 antiplasmin ELISA Kit; Alpha 2 antiplasmin pigment epithelium derived factor ELISA Kit; Alpha 2 AP ELISA Kit; ALPHA 2 PI ELISA Kit; Alpha 2 plasmin inhibitor ELISA Kit; Alpha 2 plasmin inhibitor deficiency ELISA Kit; Alpha-2-antiplasmin ELISA Kit; Alpha-2-AP ELISA Kit; Alpha-2-PI ELISA Kit; Alpha-2-plasmin inhibitor ELISA Kit; Antiplasmin deficiency ELISA Kit; API ELISA Kit; Plasmin inhibitor deficiency ELISA Kit; PLI ELISA Kit; Serine (or cysteine) peptidase inhibitor; clade F; member 2 ELISA Kit; Serine (Or cysteine) peptidase inhibitor; clade F; member 2; isoform CRA_c ELISA Kit; Serine (or cysteine) proteinase inhibitor; clade F (alpha 2 antiplasmin pigment epithelium derived factor) member 2 ELISA Kit; Serine (Or cysteine) proteinase inhibitor; clade F; member 2 ELISA Kit; Serine or cysteine peptidase inhibitor clade F member 2 ELISA Kit; Serpin F2 ELISA Kit; Serpin peptidase inhibitor clade F ELISA Kit; Serpin peptidase inhibitor; clade F (alpha 2 antiplasmin pigment epithelium derived factor) member 2 ELISA Kit; SERPINF2 ELISA Kit

缩写

SERPINF2

Uniprot No

P08697

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Others

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human α2-AP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		88
		Range %		84-93
1:2		Average %		85
		Range %		81-91
1:4		Average %		92
		Range %		88-100
1:8		Average %		95
		Range %		91-99

回收率

The recovery of human α2-AP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		89		83-94
EDTA plasma (n=4)		95		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 2.272 2.346 2.309 2.214 500 1.476 1.491 1.484 1.389 250 0.863 0.892 0.878 0.783 125 0.448 0.479 0.464 0.369 62.5 0.268 0.251 0.260 0.165 31.2 0.187 0.178 0.183 0.088 15.6 0.169 0.159 0.164 0.069 0 0.097 0.092 0.095

货期

3-5 working days