产品描述
CUSABIO's human TREM1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for quantitatively measuring human TREM1 in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of TREM1 in samples. The color intensity is measured at 450 nm via a microplate reader.
TREM1, an activating receptor expressed on myeloid cells, has the synergistic ability to amplify the signaling of the TLR4 or TLR2, which can recognize components of a variety of microorganisms including bacteria, fungi, and viruses. TREM1 mainly augments inflammation during acute inflammation and is upregulated during sepsis and pulmonary infection. It has been recognized as a critical immunomodulator in several inflammatory diseases of both infectious and non-infectious etiologies such as inflammatory bowel disease, acute pancreatitis, gouty arthritis, and atherosclerosis.
别名
CD354 ELISA Kit; OTTMUSP00000018206 ELISA Kit; TREM 1 ELISA Kit; TREM-1 ELISA Kit; TREM1 ELISA Kit; TREM1_HUMAN ELISA Kit; Triggering receptor expressed on monocytes 1 ELISA Kit; Triggering receptor expressed on myeloid cells 1 ELISA Kit; Triggering receptor TREM 1 ELISA Kit; Triggering receptor TREM1 ELISA Kit
缩写
TREM1
Uniprot No
Q9NP99
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human TREM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 97 | |
| Range % | 92-102 | |
| 1:2 | Average % | 90 | |
| Range % | 82-97 | |
| 1:4 | Average % | 104 | |
| Range % | 99-110 | |
| 1:8 | Average % | 86 | |
| Range % | 82-93 | |
回收率
| The recovery of human TREM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 84-96 | |
| EDTA plasma (n=4) | 96 | 90-101 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.529 | 2.752 | 2.641 | 2.538 | | | 1000 | 2.038 | 2.079 | 2.059 | 1.956 | | | 500 | 1.304 | 1.389 | 1.347 | 1.244 | | | 250 | 0.701 | 0.733 | 0.717 | 0.614 | | | 125 | 0.441 | 0.455 | 0.448 | 0.345 | | | 62.5 | 0.302 | 0.326 | 0.314 | 0.211 | | | 31.25 | 0.205 | 0.201 | 0.203 | 0.100 | | | 0 | 0.104 | 0.101 | 0.103 | | |
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货期
3-5 working days