产品描述

This Human SLIT3 ELISA Kit was designed for the quantitative measurement of Human SLIT3 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

别名

FLJ10764 ELISA Kit; KIAA0814 ELISA Kit; MEGF 5 ELISA Kit; MEGF5 ELISA Kit; Multiple EGF-like domains protein 5 ELISA Kit; Multiple epidermal growth factor like domains 5 ELISA Kit; Multiple epidermal growth factor-like domains protein 5 ELISA Kit; SLIL 2 ELISA Kit; SLIL2 ELISA Kit; SLIT 1 ELISA Kit; Slit 2 ELISA Kit; Slit 3 ELISA Kit; Slit homolog 3 (Drosophila) ELISA Kit; Slit homolog 3 ELISA Kit; Slit homolog 3 protein ELISA Kit; Slit-3 ELISA Kit; SLIT1 ELISA Kit; Slit2 ELISA Kit; Slit3 ELISA Kit; SLIT3_HUMAN ELISA Kit

缩写

SLIT3

Uniprot No

O75094

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Neuroscience

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human SLIT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		93
		Range %		90-97
1:2		Average %		85
		Range %		82-93
1:4		Average %		110
		Range %		105-115
1:8		Average %		106
		Range %		102-117

回收率

The recovery of human SLIT3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		86-95
EDTA plasma (n=4)		99		95-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected     10 1.866 1.955 1.911 1.777     5 1.472 1.588 1.530 1.396     2.5 1.162 1.201 1.182 1.048     1.25 0.864 0.873 0.869 0.735     0.625 0.525 0.558 0.542 0.408     0.312 0.312 0.313 0.313 0.179     0.156 0.267 0.276 0.272 0.138     0 0.137 0.131 0.134

货期

3-5 working days