产品描述
This Mouse pro-IL1B ELISA Kit was designed for the quantitative measurement of Mouse pro-IL1B protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.
缩写
pro-IL1B
灵敏度
1.56 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse pro-IL1B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 106 | | |
| Range % | 101-113 | | |
| 1:2 | Average % | 87 | | |
| Range % | 82-91 | | |
| 1:4 | Average % | 94 | | |
| Range % | 90-99 | | |
| 1:8 | Average % | 85 | | |
| Range % | 80-90 | | |
回收率
| The recovery of mouse pro-IL1B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 96 | 91-100 | | |
| EDTA plasma (n=4) | 91 | 85-97 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | | 400 | 2.496 | 2.362 | 2.429 | 2.282 | | | | 200 | 1.951 | 1.841 | 1.896 | 1.749 | | | | 100 | 1.349 | 1.319 | 1.334 | 1.187 | | | | 50 | 0.935 | 0.885 | 0.910 | 0.763 | | | | 25 | 0.603 | 0.627 | 0.615 | 0.468 | | | | 12.5 | 0.367 | 0.374 | 0.371 | 0.224 | | | | 6.25 | 0.228 | 0.236 | 0.232 | 0.085 | | | | 0 | 0.144 | 0.150 | 0.147 | | | |
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货期
3-5 working days