产品描述
RETN(全称Retinol-binding protein 4)是一种由脂肪细胞分泌的蛋白质,主要参与能量代谢和炎症反应等生理过程。其含量水平与肥胖、糖尿病、代谢综合征等疾病相关,研究表明RETN可促进胰岛素抵抗和炎症反应,从而导致多种代谢异常。因此,RETN可能成为这些疾病的治疗和预防的潜在靶点。
华美生物所提供的Human Resistin ELISA KIT属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的RETN,其灵敏度为0.078 ng/mL,检测范围为0.312 ng/mL-20 ng/mL。
别名
Adipose tissue specific secretory factor ELISA Kit; Adipose tissue-specific secretory factor ELISA Kit; ADSF ELISA Kit; C/EBP epsilon regulated myeloid specific secreted cysteine rich protein ELISA Kit; C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1 ELISA Kit; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein ELISA Kit; CG5403 ELISA Kit; Cysteine rich secreted protein A12 alpha like 2 ELISA Kit; Cysteine rich secreted protein FIZZ3 ELISA Kit; Cysteine-rich secreted protein A12-alpha-like 2 ELISA Kit; Cysteine-rich secreted protein FIZZ3 ELISA Kit; dri ELISA Kit; FIZZ 3 ELISA Kit; FIZZ3 ELISA Kit; Found in inflammatory zone 3 ELISA Kit; HXCP 1 ELISA Kit; HXCP1 ELISA Kit; MGC126603 ELISA Kit; MGC126609 ELISA Kit; PRO1199 ELISA Kit; Resistin ELISA Kit; Resistin delta2 ELISA Kit; RETN 1 ELISA Kit; RETN ELISA Kit; RETN_HUMAN ELISA Kit; RETN1 ELISA Kit; RSTN ELISA Kit; UNQ407 ELISA Kit; XCP 1 ELISA Kit; XCP1 ELISA Kit
缩写
RETN
Uniprot No
Q9HD89
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human resistin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
97 |
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| Range % |
92-103 |
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| 1:2 |
Average % |
94 |
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| Range % |
91-98 |
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| 1:4 |
Average % |
102 |
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| Range % |
98-106 |
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| 1:8 |
Average % |
86 |
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| Range % |
80-92 |
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回收率
| The recovery of human resistin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
90-104 |
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| EDTA plasma (n=4) |
92 |
88-97 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.285 |
2.330 |
2.308 |
2.204 |
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| 10 |
1.350 |
1.427 |
1.389 |
1.285 |
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| 5 |
0.701 |
0.733 |
0.717 |
0.613 |
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| 2.5 |
0.400 |
0.424 |
0.412 |
0.308 |
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| 1.25 |
0.256 |
0.268 |
0.262 |
0.158 |
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| 0.625 |
0.178 |
0.169 |
0.174 |
0.070 |
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| 0.312 |
0.132 |
0.129 |
0.131 |
0.027 |
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| 0 |
0.105 |
0.102 |
0.104 |
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货期
3-5 working days