产品描述

This Human PEDF ELISA Kit was designed for the quantitative measurement of Human PEDF protein in serum, cell culture supernates, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

别名

Cell proliferation-inducing gene 35 protein ELISA Kit; EPC 1 ELISA Kit; EPC-1 ELISA Kit; EPC1 ELISA Kit; OI12 ELISA Kit; OI6 ELISA Kit; PEDF ELISA Kit; PEDF_HUMAN ELISA Kit; PIG 35 ELISA Kit; PIG35 ELISA Kit; Pigment epithelium derived factor ELISA Kit; Pigment epithelium-derived factor ELISA Kit; Proliferation inducing protein 35 ELISA Kit; Serine (or cysteine) proteinase inhibitor ELISA Kit; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 ELISA Kit; Serpin F1 ELISA Kit; Serpin family F member 1 ELISA Kit; Serpin peptidase inhibitor ELISA Kit; Serpin peptidase inhibitor clade F member 1 ELISA Kit; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 ELISA Kit; SERPINF 1 ELISA Kit; Serpinf1 ELISA Kit

缩写

PEDF

Uniprot No

P36955

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PEDF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		100
		Range %		96-104
1:2		Average %		95
		Range %		89-104
1:4		Average %		96
		Range %		91-103
1:8		Average %		92
		Range %		88-97

回收率

The recovery of human PEDF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		91		85-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 10 2.535 2.575 2.555 2.441 5 2.012 2.032 2.022 1.908 2.5 1.398 1.428 1.413 1.299 1.25 0.847 0.867 0.857 0.743 0.625 0.478 0.498 0.488 0.374 0.312 0.336 0.346 0.341 0.227 0.156 0.237 0.250 0.244 0.130 0 0.109 0.118 0.114

货期

3-5 working days