产品描述

This Human SERPINA1 ELISA Kit was designed for the quantitative measurement of Human SERPINA1 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 78 nIU/mL-5000 nIU/mL and the sensitivity is 19.5 nIU/mL.

别名

A1A ELISA Kit; A1AT ELISA Kit; A1AT_HUMAN ELISA Kit; AAT ELISA Kit; Alpha 1 antiproteinase ELISA Kit; Alpha 1 antitrypsin ELISA Kit; Alpha 1 antitrypsin null ELISA Kit; Alpha 1 protease inhibitor ELISA Kit; Alpha-1 protease inhibitor ELISA Kit; Alpha-1-antiproteinase ELISA Kit; alpha1 proteinase inhibitor ELISA Kit; Alpha1AT ELISA Kit; Dom1 ELISA Kit; PI ELISA Kit; PI1 ELISA Kit; PRO0684 ELISA Kit; PRO2275 ELISA Kit; Serine (or cysteine) proteinase inhibitor clade A member 1 ELISA Kit; Serine protease inhibitor 1-1 ELISA Kit; Serine protease inhibitor A1a ELISA Kit; Serpin A1 ELISA Kit; Serpin A1a ELISA Kit; Serpin peptidase inhibitor clade A member 1 ELISA Kit; Serpina1 ELISA Kit; Short peptide from AAT ELISA Kit; SPAAT ELISA Kit; Spi1-1 ELISA Kit

缩写

SERPINA1

Uniprot No

P01009

灵敏度

19.5 nIU/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human α1-AT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1000		Average %		86
		Range %		80-92
1:2000		Average %		99
		Range %		91-105
1:4000		Average %		103
		Range %		92-110
1:8000		Average %		93
		Range %		86-98

回收率

The recovery of human α1-AT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		93		89-98
EDTA plasma (n=4)		95		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

nIU/ml OD1 OD2 Average Corrected 5000 2.943 2.903 2.923 2.797 2500 2.296 2.201 2.249 2.123 1250 1.633 1.589 1.611 1.485 625 0.927 0.989 0.958 0.832 312 0.533 0.521 0.527 0.401 156 0.348 0.334 0.341 0.215 78 0.256 0.236 0.246 0.120 0 0.130 0.122 0.126

货期

3-5 working days