产品描述
This Rat TCC C5b9 ELISA Kit was designed for the quantitative measurement of Rat TCC C5b9 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL .
缩写
TCC C5b9
灵敏度
0.39 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:100 | Average % | 91 | |
| Range % | 86-98 | |
| 1:200 | Average % | 96 | |
| Range % | 92-102 | |
| 1:400 | Average % | 85 | |
| Range % | 81-88 | |
| 1:800 | Average % | 88 | |
| Range % | 83-95 | |
回收率
| The recovery of rat TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 90-98 | |
| EDTA plasma (n=4) | 90 | 87-95 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 100 | 2.351 | 2.502 | 2.427 | 2.282 | | | 50 | 2.021 | 2.165 | 2.093 | 1.948 | | | 25 | 1.423 | 1.540 | 1.482 | 1.337 | | | 12.5 | 0.936 | 1.002 | 0.969 | 0.824 | | | 6.25 | 0.607 | 0.581 | 0.594 | 0.449 | | | 3.12 | 0.372 | 0.360 | 0.366 | 0.221 | | | 1.56 | 0.238 | 0.257 | 0.248 | 0.103 | | | 0 | 0.141 | 0.149 | 0.145 | | |
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货期
3-5 working days