产品描述
The Human Platelet Activating Factor (PAF) ELISA Kit is a powerful tool for quantitative detection of PAF in various sample types. PAF, also known as a platelet-activating factor, is a bioactive lipid mediator that plays a crucial role in inflammation and immune response.
This kit is designed for the detection of human PAF in serum, urine, cell culture supernates, tissue homogenates, and cell lysates. Based on a sandwich assay principle, our kit provides accurate and reproducible results with minimal interference from other proteins.
With a sensitivity of 1.17 ng/mL and a detection range of 4.69 ng/mL-300 ng/mL, this ELISA kit can accurately measure PAF levels in various sample types. The assay time ranges from 1-5 hours, and the sample volume required is 50-100ul. The detection wavelength is 450 nm, ensuring accurate and precise measurement of PAF levels.
This PAF ELISA Kit has been cited in more than 8 publications, demonstrating its effectiveness and reliability in research studies. It is an essential tool for researchers studying the role of PAF in various diseases, such as asthma, sepsis, and cardiovascular disease.
别名
N/A
缩写
PAF
灵敏度
1.17 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Biochemicals
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human PAF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 85 | |
| Range % | 81-92 | |
| 1:2 | Average % | 95 | |
| Range % | 90-105 | |
| 1:4 | Average % | 94 | |
| Range % | 91-102 | |
| 1:8 | Average % | 90 | |
| Range % | 80-98 | |
回收率
| The recovery of human PAF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 91 | 88-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 300 | 2.097 | 2.093 | 2.095 | 1.994 | | | 150 | 1.823 | 1.793 | 1.808 | 1.707 | | | 75 | 1.476 | 1.374 | 1.425 | 1.324 | | | 37.5 | 1.029 | 0.986 | 1.008 | 0.907 | | | 18.75 | 0.782 | 0.772 | 0.777 | 0.676 | | | 9.38 | 0.421 | 0.401 | 0.411 | 0.310 | | | 4.69 | 0.218 | 0.202 | 0.210 | 0.109 | | | 0 | 0.105 | 0.097 | 0.101 | | |
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货期
3-5 working days
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