产品描述
This Mouse IL3 ELISA Kit was designed for the quantitative measurement of Mouse IL3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.
别名
Il3 ELISA Kit; Csfmu ELISA Kit; Il-3 ELISA Kit; Interleukin-3 ELISA Kit; IL-3 ELISA Kit; Hematopoietic growth factor ELISA Kit; Mast cell growth factor ELISA Kit; MCGF ELISA Kit; Multipotential colony-stimulating factor ELISA Kit; P-cell-stimulating factor ELISA Kit
缩写
IL3
Uniprot No
P01586
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 88 | | |
| Range % | 83-92 | | |
| 1:2 | Average % | 95 | | |
| Range % | 91-100 | | |
| 1:4 | Average % | 90 | | |
| Range % | 86-99 | | |
| 1:8 | Average % | 95 | | |
| Range % | 86-98 | | |
回收率
| The recovery of mouse IL-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 94 | 90-98 | | |
| EDTA plasma (n=4) | 97 | 86-100 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | | 500 | 2.695 | 2.680 | 2.688 | 2.511 | | | | 250 | 1.885 | 1.867 | 1.876 | 1.699 | | | | 125 | 1.302 | 1.295 | 1.299 | 1.122 | | | | 62.5 | 0.758 | 0.744 | 0.751 | 0.574 | | | | 31.2 | 0.499 | 0.492 | 0.496 | 0.319 | | | | 15.6 | 0.356 | 0.359 | 0.358 | 0.181 | | | | 7.8 | 0.281 | 0.287 | 0.284 | 0.107 | | | | 0 | 0.175 | 0.179 | 0.177 | | | |
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货期
3-5 working days