产品描述
This Rat MMP13 ELISA Kit was designed for the quantitative measurement of Rat MMP13 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 93.75 pg/mL-6000 pg/mL and the sensitivity is 23.44 pg/mL .
别名
Mmp13 ELISA Kit; Collagenase 3 ELISA Kit; EC 3.4.24.- ELISA Kit; Matrix metalloproteinase-13 ELISA Kit; MMP-13 ELISA Kit; UMRCASE ELISA Kit; Fragment ELISA Kit
缩写
MMP13
Uniprot No
P23097
灵敏度
23.44 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 86 | |
| Range % | 83-90 | |
| 1:2 | Average % | 94 | |
| Range % | 90-98 | |
| 1:4 | Average % | 98 | |
| Range % | 93-104 | |
| 1:8 | Average % | 102 | |
| Range % | 98-105 | |
回收率
| The recovery of rat MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-99 | |
| EDTA plasma (n=4) | 90 | 85-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 6000 | 2.231 | 2.269 | 2.250 | 2.138 | | | 3000 | 1.526 | 1.539 | 1.533 | 1.421 | | | 1500 | 0.974 | 0.997 | 0.986 | 0.874 | | | 750 | 0.578 | 0.596 | 0.587 | 0.475 | | | 375 | 0.345 | 0.327 | 0.336 | 0.224 | | | 187.5 | 0.251 | 0.241 | 0.246 | 0.134 | | | 93.75 | 0.185 | 0.189 | 0.187 | 0.075 | | | 0 | 0.111 | 0.113 | 0.112 | | |
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货期
3-5 working days