产品描述
IL6 (Interleukin-6) 是一种细胞因子,在免疫反应、炎症反应、细胞增殖和分化等生物过程中发挥重要作用。它能够刺激机体免疫细胞的生长、增殖和分化,并调节机体的免疫反应,其高含量与多种炎症疾病的发生和发展相关。
华美生物所提供的Bovine Interleukin 6,IL-6 ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测牛血清、血浆、组织匀浆样本中的IL6,其灵敏度为2.5 pg/mL,检测范围为5 pg/mL-1000 pg/mL。
别名
IL6 Interleukin-6 ELISA Kit; IL-6 ELISA Kit
缩写
IL6
Uniprot No
P26892
灵敏度
2.5 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
90-97 |
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| 1:2 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:4 |
Average % |
95 |
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| Range % |
88-101 |
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| 1:8 |
Average % |
95 |
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| Range % |
90-100 |
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回收率
| The recovery of bovine IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
94-101 |
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| EDTA plasma (n=4) |
96 |
92-103 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 1000 |
0.071 |
0.075 |
0.073 |
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| 250 |
0.192 |
0.205 |
0.199 |
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| 80 |
0.421 |
0.451 |
0.436 |
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| 20 |
0.876 |
0.897 |
0.887 |
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| 5 |
1.345 |
1.316 |
1.331 |
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| 0 |
2.116 |
2.215 |
2.166 |
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货期
3-5 working days