产品描述
This Dog MMP2/Gelatinase A ELISA Kit was designed for the quantitative measurement of Dog MMP2/Gelatinase A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.69 ng/mL-300 ng/mL and the sensitivity is 1.17 ng/mL .
缩写
MMP2/Gelatinase A
Uniprot No
F1PMK7
灵敏度
1.17 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of canine MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 98 | |
| Range % | 94-106 | |
| 1:2 | Average % | 85 | |
| Range % | 80-95 | |
| 1:4 | Average % | 95 | |
| Range % | 92-100 | |
| 1:8 | Average % | 93 | |
| Range % | 86-98 | |
回收率
| The recovery of canine MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 90-101 | |
| EDTA plasma (n=4) | 92 | 87-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | 300 | 2.028 | 1.952 | 1.990 | 1.854 | | | 150 | 1.736 | 1.776 | 1.756 | 1.620 | | | 75 | 1.502 | 1.452 | 1.477 | 1.341 | | | 37.5 | 1.128 | 1.139 | 1.134 | 0.998 | | | 18.75 | 0.757 | 0.785 | 0.771 | 0.635 | | | 9.38 | 0.503 | 0.524 | 0.514 | 0.378 | | | 4.69 | 0.331 | 0.329 | 0.330 | 0.194 | | | 0 | 0.137 | 0.134 | 0.136 | | |
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货期
3-5 working days