产品描述
This Chicken IL17 ELISA Kit was designed for the quantitative measurement of Chicken IL17 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 150 pg/mL-6000 pg/mL and the sensitivity is 150 pg/mL.
缩写
IL-17
Uniprot No
Q7T1P7
灵敏度
150 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
82-100 |
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| 1:2 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:4 |
Average % |
96 |
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| Range % |
90-101 |
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| 1:8 |
Average % |
91 |
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| Range % |
85-98 |
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回收率
| The recovery of chicken IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
91 |
85-96 |
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| EDTA plasma (n=4) |
92 |
86-98 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 0 |
1.914 |
2.027 |
1.971 |
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| 150 |
1.306 |
1.329 |
1.318 |
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| 450 |
1.039 |
1.102 |
1.071 |
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| 1380 |
0.755 |
0.702 |
0.729 |
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| 3000 |
0.484 |
0.462 |
0.473 |
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| 6000 |
0.351 |
0.348 |
0.350 |
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货期
3-5 working days