产品描述
This Mouse PDCD1 ELISA Kit was designed for the quantitative measurement of Mouse PDCD1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
别名
Pdcd1 ELISA Kit; Pd1 ELISA Kit; Programmed cell death protein 1 ELISA Kit; Protein PD-1 ELISA Kit; mPD-1 ELISA Kit; CD antigen CD279 ELISA Kit
缩写
PDCD1
Uniprot No
Q02242
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PD-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 97 | | |
| Range % | 92-101 | | |
| 1:2 | Average % | 91 | | |
| Range % | 85-96 | | |
| 1:4 | Average % | 84 | | |
| Range % | 80-88 | | |
| 1:8 | Average % | 95 | | |
| Range % | 95-99 | | |
回收率
| The recovery of mouse PD-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 101 | 96-107 | | |
| EDTA plasma (n=4) | 95 | 90-100 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | | 20 | 2.652 | 2.528 | 2.590 | 2.430 | | | | 10 | 2.121 | 2.009 | 2.065 | 1.905 | | | | 5 | 1.439 | 1.417 | 1.428 | 1.268 | | | | 2.5 | 0.994 | 0.952 | 0.973 | 0.813 | | | | 1.25 | 0.651 | 0.680 | 0.666 | 0.506 | | | | 0.625 | 0.384 | 0.375 | 0.380 | 0.220 | | | | 0.312 | 0.256 | 0.261 | 0.259 | 0.099 | | | | 0 | 0.163 | 0.156 | 0.160 | | | |
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货期
3-5 working days