产品描述
LPS (Lipopolysaccharide) 是一种内毒素,存在于许多革兰阴性菌的细胞外膜中,它的存在对于细菌的生长和生存是必要的。LPS的主要作用是激活免疫系统,引起免疫细胞的炎症反应,同时也可以引发严重的炎症反应和休克。在体内,LPS可以通过血液-脑屏障和血液-脊髓液屏障进入中枢神经系统,引起神经炎症反应和神经系统损伤。
华美生物所提供的Human Lipopolysaccharides,LPS ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的LPS,其灵敏度为1.56 pg/mL,检测范围为6.25 pg/mL-400 pg/mL。
缩写
LPS
灵敏度
1.56 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human LPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
82-94 |
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| 1:2 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:4 |
Average % |
92 |
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| Range % |
87-96 |
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| 1:8 |
Average % |
94 |
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| Range % |
89-99 |
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回收率
| The recovery of human LPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
90-97 |
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| EDTA plasma (n=4) |
88 |
82-96 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.809 |
2.960 |
2.885 |
2.720 |
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| 200 |
2.227 |
2.366 |
2.297 |
2.132 |
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| 100 |
1.515 |
1.556 |
1.536 |
1.371 |
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| 50 |
0.789 |
0.755 |
0.772 |
0.607 |
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| 25 |
0.432 |
0.470 |
0.451 |
0.286 |
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| 12.5 |
0.303 |
0.325 |
0.314 |
0.149 |
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| 6.25 |
0.235 |
0.232 |
0.234 |
0.069 |
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| 0 |
0.164 |
0.165 |
0.165 |
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货期
3-5 working days