产品描述

This Human KLK2 ELISA Kit was designed for the quantitative measurement of Human KLK2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

别名

Glandular kallikrein 2 ELISA Kit; Glandular kallikrein-1 ELISA Kit; hGK 1 ELISA Kit; hGK-1 ELISA Kit; hK2 ELISA Kit; Kallikrein 2 prostatic ELISA Kit; Kallikrein related peptidase 2 ELISA Kit; kallikrein, glandular ELISA Kit; Kallikrein, prostatic ELISA Kit; Kallikrein-2 ELISA Kit; KLK 2 ELISA Kit; Klk-2 ELISA Kit; Klk2 ELISA Kit; KLK2_HUMAN ELISA Kit; KLK2A 2 ELISA Kit; KLK2A2 ELISA Kit; MGC12201 ELISA Kit; Tissue kallikrein 2 ELISA Kit; Tissue kallikrein-2 ELISA Kit; Ton ELISA Kit

缩写

KLK2

Uniprot No

P20151

灵敏度

15.6 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human hK 2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		87
		Range %		82-91
1:2		Average %		94
		Range %		89-98
1:4		Average %		97
		Range %		94-107
1:8		Average %		92
		Range %		88-96

回收率

The recovery of human hK 2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		91-99
EDTA plasma (n=4)		103		98-108

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 4000 2.371 2.201 2.286 2.180 2000 1.973 1.921 1.947 1.841 1000 1.312 1.405 1.359 1.253 500 0.712 0.732 0.722 0.616 250 0.337 0.342 0.340 0.234 125 0.256 0.261 0.259 0.153 62.5 0.197 0.199 0.198 0.092 0 0.105 0.107 0.106

货期

3-5 working days