产品描述
This Goat IL2 ELISA Kit was designed for the quantitative measurement of Goat IL2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
IL2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit
缩写
IL2
Uniprot No
P36835
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 90 | |
| Range % | 85-95 | |
| 1:2 | Average % | 98 | |
| Range % | 94-102 | |
| 1:4 | Average % | 102 | |
| Range % | 94-107 | |
| 1:8 | Average % | 94 | |
| Range % | 87-98 | |
回收率
| The recovery of goat IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 95-102 | |
| EDTA plasma (n=4) | 93 | 88-97 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.491 | 2.365 | 2.428 | 2.340 | | | 500 | 1.566 | 1.594 | 1.580 | 1.492 | | | 250 | 1.030 | 1.096 | 1.063 | 0.975 | | | 125 | 0.697 | 0.753 | 0.725 | 0.637 | | | 62.5 | 0.423 | 0.443 | 0.433 | 0.345 | | | 31.2 | 0.256 | 0.277 | 0.267 | 0.179 | | | 15.6 | 0.168 | 0.169 | 0.169 | 0.081 | | | 0 | 0.087 | 0.089 | 0.088 | | |
|
货期
3-5 working days