产品描述
This Rat MAP2 ELISA Kit was designed for the quantitative measurement of Rat MAP2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .
别名
Map2 ELISA Kit; Mtap2 ELISA Kit; Microtubule-associated protein 2 ELISA Kit; MAP-2 ELISA Kit
缩写
MAP2
Uniprot No
P15146
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MAP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 90 | |
| Range % | 86-93 | |
| 1:2 | Average % | 103 | |
| Range % | 98-105 | |
| 1:4 | Average % | 93 | |
| Range % | 88-97 | |
| 1:8 | Average % | 97 | |
| Range % | 91-101 | |
回收率
| The recovery of rat MAP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 91-98 | |
| EDTA plasma (n=4) | 97 | 92-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.329 | 2.312 | 2.321 | 2.185 | | | 5 | 1.844 | 1.776 | 1.810 | 1.674 | | | 2.5 | 1.274 | 1.296 | 1.285 | 1.149 | | | 1.25 | 0.923 | 0.917 | 0.920 | 0.784 | | | 0.625 | 0.634 | 0.624 | 0.629 | 0.493 | | | 0.312 | 0.433 | 0.443 | 0.438 | 0.302 | | | 0.156 | 0.297 | 0.282 | 0.290 | 0.154 | | | 0 | 0.138 | 0.134 | 0.136 | | |
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货期
3-5 working days