产品描述

This human LGALS9 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human LGALS9 in different samples, including serum, plasma, cell culture supernates, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of LGALS9 bound in the initial step.

LGALS9 is secreted out upon cell damage and acts as an apoptosis-inducing factor that binds to TIM-3 on Th1 cells. It elicits a set of events such as calcium mobilization, calpain and caspase-1 activation that drives the Th1 cells to apoptosis. It has been involved in multiple biological processes, including glycoprotein trafficking, cell-cell or cell-extracellular matrix interaction, signal transduction, immune response, development, and oncogenesis. LGALS9 modulates the threshold of B cell activation. Engagement of LGALS9 alters the organization of the B cell receptor (BCR), boosting interactions with inhibitory co-receptors CD45 and CD22, thereby reducing BCR signal transduction.

别名

36 kDa beta-galactoside-binding lectin ELISA Kit; Ecalectin ELISA Kit; Gal-9 ELISA Kit; galectin 9 ELISA Kit; Galectin-9 ELISA Kit; galectin9 ELISA Kit; HOM HD 21 ELISA Kit; HOMHD21 ELISA Kit; HUAT ELISA Kit; Lectin galactoside binding soluble 9 ELISA Kit; LEG9_HUMAN ELISA Kit; LGAL S9 ELISA Kit; LGALS 9 ELISA Kit; Lgals9 ELISA Kit; LGALS9A ELISA Kit; MGC117375 ELISA Kit; MGC125973 ELISA Kit; MGC125974 ELISA Kit; Tumor antigen HOM-HD-21 ELISA Kit; UAT ELISA Kit; Urate transporter/channel ELISA Kit; Urate transporter/channel protein ELISA Kit

缩写

LGALS9

Uniprot No

O00182

灵敏度

7.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human LGALS9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		86-97
1:2		Average %		100
		Range %		95-104
1:4		Average %		88
		Range %		83-94
1:8		Average %		94
		Range %		87-99

回收率

The recovery of human LGALS9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		88		83-93
EDTA plasma (n=4)		96		92-101

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   2000 1.987 1.952 1.970 1.814   1000 1.345 1.376 1.361 1.205   500 0.942 0.904 0.923 0.767   250 0.553 0.539 0.546 0.390   125 0.355 0.372 0.364 0.208   62.5 0.261 0.277 0.269 0.113   31.25 0.202 0.189 0.196 0.040   0 0.157 0.155 0.156

货期

3-5 working days