产品描述
ORM2(orosomucoid 2)是一种血清糖蛋白,通常由肝脏合成。ORM2可以调节和减少细胞因子和白细胞趋化因子的作用。ORM2的含量在肝脏疾病、炎症和感染时常常会发生变化,因此可以作为这些疾病的生物标志物。
华美生物所提供的Human orosomucoid 2,ORM2 ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测人血清、血浆、组织匀浆样本中的ORM2,其灵敏度为19.5 ng/mL,检测范围为78 ng/mL-5000 ng/mL。
别名
ORM2 ELISA Kit; AGP2Alpha-1-acid glycoprotein 2 ELISA Kit; AGP 2 ELISA Kit; Orosomucoid-2 ELISA Kit; OMD 2 ELISA Kit
缩写
ORM2
Uniprot No
P19652
灵敏度
19.5 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human ORM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
98 |
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| Range % |
90-102 |
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| 1:200 |
Average % |
89 |
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| Range % |
83-94 |
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| 1:400 |
Average % |
101 |
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| Range % |
93-105 |
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| 1:800 |
Average % |
105 |
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| Range % |
97-110 |
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回收率
| The recovery of human ORM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
82-95 |
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| EDTA plasma (n=4) |
94 |
90-98 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 5000 |
0.131 |
0.135 |
0.133 |
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| 2500 |
0.389 |
0.401 |
0.395 |
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| 1250 |
0.750 |
0.732 |
0.741 |
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| 625 |
1.042 |
1.020 |
1.031 |
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| 312 |
1.441 |
1.454 |
1.448 |
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| 156 |
1.650 |
1.666 |
1.658 |
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| 78 |
1.806 |
1.767 |
1.787 |
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| 0 |
2.001 |
2.078 |
2.040 |
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货期
3-5 working days