产品描述

The human TIEG1 Elisa kit is suitable for the quantitative measurement of human TIEG1 in serum, plasma, tissue homogenates, or cell lysates. This assay employs the sandwich enzyme immunoassay technique and enzyme-substrate chromogenic reaction. The color develops positively to the amount of human TIEG1 in samples. The color development is stopped and the intensity of the color is measured. This kit displays many advantages, including high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.

TIEG1, also known as KLF10, is inducibly expressed by TGF-β, bone morphogenetic proteins (BMPs), and other cytokines. It is expressed in specific cell types in numerous tissues and is involved in inhibiting cell proliferation and inflammation as well as inducing apoptosis similar to that of TGFβ. TIEG1 binds to Sp-1-GC-rich DNA sequences and can activate or suppress the transcription of numerous genes. TIEG1 also plays an important role in regulating cell apoptosis and proliferation in various diseases, including pancreatic cancer, leukemia, and osteoporosis.

别名

Early growth response; alpha ELISA Kit; Early growth response; alpha like ELISA Kit; EGR A ELISA Kit; EGR alpha ELISA Kit; EGR(A) ELISA Kit; EGR-alpha ELISA Kit; EGRA ELISA Kit; Egral ELISA Kit; EGRalpha ELISA Kit; Gdnfif ELISA Kit; KLF 10 ELISA Kit; KLF10 ELISA Kit; KLF10_HUMAN ELISA Kit; Krueppel like factor 10 ELISA Kit; Krueppel like factor10 ELISA Kit; Krueppel-like factor 10 ELISA Kit; Kruppel like factor 10 ELISA Kit; mGIF ELISA Kit; TGF-beta- inducible early gene ELISA Kit; TGFB inducible early growth response 1 ELISA Kit; TGFB inducible early growth response ELISA Kit; TGFB inducible early growth response protein 1 ELISA Kit; TGFB-inducible early growth response protein 1 ELISA Kit; TIEG 1 ELISA Kit; TIEG ELISA Kit; TIEG-1 ELISA Kit; TIEG1 ELISA Kit; Transforming growth factor beta inducible early growth response ELISA Kit; Transforming growth factor beta inducible early growth response protein 1 ELISA Kit; Transforming growth factor-beta-inducible early growth response protein 1 ELISA Kit; Zinc finger transcription factor TIEG ELISA Kit

缩写

KLF10

Uniprot No

Q13118

灵敏度

8.6 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human TIEG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		85
		Range %		81-89
1:2		Average %		93
		Range %		90-96
1:4		Average %		84
		Range %		81-86
1:8		Average %		95
		Range %		91-99

回收率

The recovery of human TIEG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		103		100-105
EDTA plasma (n=4)		98		94-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 2200 2.435 2.431 2.433 2.310 1100 1.708 1.687 1.698 1.575 550 1.020 1.000 1.010 0.887 275 0.533 0.570 0.552 0.429 137.5 0.361 0.358 0.360 0.237 68.75 0.291 0.287 0.289 0.166 34.38 0.203 0.212 0.208 0.085 0 0.122 0.124 0.123

货期

3-5 working days