产品描述
This Mouse IGFBP1 ELISA Kit was designed for the quantitative measurement of Mouse IGFBP1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
别名
Igfbp1 ELISA Kit; Igfbp-1 ELISA Kit; Insulin-like growth factor-binding protein 1 ELISA Kit; IBP-1 ELISA Kit; IGF-binding protein 1 ELISA Kit; IGFBP-1 ELISA Kit
缩写
IGFBP1
Uniprot No
P47876
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IGFBP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:100 | Average % | 89 | | |
| Range % | 85-94 | | |
| 1:200 | Average % | 102 | | |
| Range % | 96-107 | | |
| 1:400 | Average % | 92 | | |
| Range % | 87-98 | | |
| 1:800 | Average % | 94 | | |
| Range % | 89-98 | | |
回收率
| The recovery of mouse IGFBP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 98 | 93-102 | | |
| EDTA plasma (n=4) | 103 | 92-107 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | | 20 | 2.179 | 2.234 | 2.207 | 2.081 | | | | 10 | 1.816 | 1.943 | 1.880 | 1.754 | | | | 5 | 1.337 | 1.345 | 1.341 | 1.215 | | | | 2.5 | 0.839 | 0.846 | 0.843 | 0.717 | | | | 1.25 | 0.405 | 0.412 | 0.409 | 0.283 | | | | 0.625 | 0.278 | 0.265 | 0.272 | 0.146 | | | | 0.312 | 0.182 | 0.189 | 0.186 | 0.060 | | | | 0 | 0.125 | 0.126 | 0.126 | | | |
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货期
3-5 working days