产品描述

This human IL-37 ELISA Kit is suitable for qualitatively determining human concentrations in multiple biological fluids, including human serum, plasma, and tissue homogenates in vitro. IL-37 plays an important role in innate and adaptive immunity and may be a useful molecule for effective cytokine therapy. During inflammation, IL-37 inhibits the expression of several pro-inflammatory cytokines to facilitate the expression of the anti-inflammatory ones by regulating macrophage polar. In addition to modulating innate and acquired immunity, IL-37 also improves aging-associated immunosenescence. Furthermore, IL-37 exhibits an inhibitory effect on tumor angiogenesis and metastasis and progression.

This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of human IL-37 in the sample. Standards and samples are respectively added to the microplate wells pre-coated with an anti-human IL-37 antibody. Biotin-labeled IL-37 antibody, HRP-avidin, and TMB substrate are pipped into the microplate in turn. The capture antibody pre-coated on the plate captures the IL-37n in the human samples. IL-37 binds to the biotinylated anti-IL-37 human monoclonal antibody. And the biotin on the biotinylated anti-IL-37 human monoclonal antibody binds to the avidin on the enzyme label, forming immune complexes. The color renders blue after the addition of the TMB substrate. The addition of the stop solution into the wells immediately turns the blue into yellow. The concentration of IL-37 in the samples is directly proportional to OD (450nm). Each manufactured lot of this ELISA kit was quality tested for criteria such as sensitivity, specificity, precision, linearity, and lot-to-lot consistency.

别名

FIL1 ELISA Kit; FIL1 zeta ELISA Kit; FIL1(ZETA) ELISA Kit; FIL1Z ELISA Kit; IL 1 zeta ELISA Kit; IL 1F7 ELISA Kit; IL 1F7b (IL 1H4; IL 1H; IL 1RP1) ELISA Kit; IL 1H4 ELISA Kit; IL 1RP1 ELISA Kit; IL 1X ELISA Kit; IL 1X protein ELISA Kit; IL-1 zeta ELISA Kit; IL-1F7 ELISA Kit; IL-1H ELISA Kit; IL-1H4 ELISA Kit; IL-1RP1 ELISA Kit; IL-1X ELISA Kit; IL-37 ELISA Kit; IL1F7 (canonical product IL 1F7b) ELISA Kit; IL1F7 ELISA Kit; IL1F7_HUMAN ELISA Kit; IL1H4 ELISA Kit; IL1RP1 ELISA Kit; Interleukin 1 family member 7 ELISA Kit; interleukin 1 family; member 7 (zeta) ELISA Kit; Interleukin 1 homolog 4 ELISA Kit; Interleukin 1 related protein ELISA Kit; Interleukin 1 superfamily z ELISA Kit; Interleukin 1 zeta ELISA Kit; Interleukin 37 ELISA Kit; Interleukin-1 family member 7 ELISA Kit; Interleukin-1 homolog 4 ELISA Kit; Interleukin-1 zeta ELISA Kit; Interleukin-1-related protein ELISA Kit; Interleukin-23 ELISA Kit

缩写

IL1F7

Uniprot No

Q9NZH6

灵敏度

7.81 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-37 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

87

Range %

81-93

1:2

Average %

85

Range %

80-92

1:4

Average %

95

Range %

90-100

1:8

Average %

98

Range %

88-103

回收率

The recovery of human IL-37 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery					Range
Serum (n=5)		92					86-100
EDTA plasma (n=4)		94					84-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 2000 1.654 1.605 1.630 1.468 1000 1.219 1.233 1.226 1.064 500 0.854 0.892 0.873 0.711 250 0.562 0.581 0.572 0.410 125 0.359 0.379 0.369 0.207 62.5 0.268 0.280 0.274 0.112 31.25 0.218 0.213 0.216 0.054 0 0.165 0.159 0.162

货期

3-5 working days