产品描述
IL17A(Interleukin-17A),又称为CTLAF-7 (Cytotoxic T-lymphocyte-associated factor 7)或者是IL-17,是一种细胞因子,由Th17淋巴细胞分泌,是一种免疫系统中的重要炎症介质。IL17A能够吸引和活化中性粒细胞、T淋巴细胞和单核细胞,同时还能刺激某些组织细胞产生其他炎症介质。人体内IL17A的含量与炎症反应及某些自身免疫性疾病的发生密切相关。
华美生物所提供的Rat Interleukin 17,IL-17 ELISA KIT属于ELISA检测试剂盒,采用双抗夹心法定量检测大鼠血清、血浆、组织匀浆样本中的IL17A,其灵敏度为23.43 pg/mL,检测范围为93.75 pg/mL-6000 pg/mL。
别名
Il17a ELISA Kit; Ctla8 ELISA Kit; Il17Interleukin-17A ELISA Kit; IL-17 ELISA Kit; IL-17A ELISA Kit; Cytotoxic T-lymphocyte-associated antigen 8 ELISA Kit; CTLA-8 ELISA Kit
缩写
IL17A
Uniprot No
Q61453
灵敏度
23.43 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
80-95 |
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| 1:2 |
Average % |
85 |
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| Range % |
80-95 |
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| 1:4 |
Average % |
90 |
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| Range % |
86-100 |
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| 1:8 |
Average % |
92 |
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| Range % |
86-97 |
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回收率
| The recovery of rat IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
85-98 |
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| EDTA plasma (n=4) |
93 |
88-101 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 6000 |
1.865 |
1.802 |
1.834 |
1.739 |
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| 3000 |
1.418 |
1.376 |
1.397 |
1.302 |
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| 1500 |
1.036 |
1.004 |
1.020 |
0.925 |
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| 750 |
0.624 |
0.589 |
0.607 |
0.512 |
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| 375 |
0.379 |
0.364 |
0.372 |
0.277 |
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| 187.5 |
0.211 |
0.209 |
0.210 |
0.115 |
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| 93.75 |
0.132 |
0.122 |
0.127 |
0.032 |
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| 0 |
0.099 |
0.091 |
0.095 |
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货期
3-5 working days