产品描述
This mouse IL-23 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of mouse IL-23 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the IL-23 levels in the sample and is measured at 450 nm through a microplate reader.
IL-23 is mainly produced by macrophages and dendritic cells, in response to exogenous or endogenous signals, and drives the differentiation and activation of Th17 cells with subsequent production of IL-17A, IL-17F, IL-6, IL-22, and TNF-α. IL-23 signals through its heterodimeric receptor complex consisting of the two subunits IL-12Rβ1 and IL-23R, activating P38/MAPK, PI3K-Akt, or the NF-кB pathways. Although IL-23 plays a pivotal role in the protective immune response to bacterial and fungal infections, its dysregulation has been shown to exacerbate chronic immune-mediated inflammation. IL-23 is involved in the pathogenesis of Crohn’s Disease, Psoriasis, and Psoriatic Arthritis.
别名
N/A
缩写
IL-23
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
81-92 |
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| 1:2 |
Average % |
95 |
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| Range % |
89-100 |
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| 1:4 |
Average % |
93 |
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| Range % |
84-97 |
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| 1:8 |
Average % |
106 |
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| Range % |
97-110 |
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回收率
| The recovery of mouse IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
104 |
94-108 |
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| EDTA plasma (n=4) |
101 |
94-104 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 500 |
2.868 |
2.689 |
2.779 |
2.654 |
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| 250 |
1.519 |
1.586 |
1.553 |
1.428 |
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| 125 |
0.746 |
0.700 |
0.723 |
0.598 |
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| 62.5 |
0.434 |
0.456 |
0.445 |
0.320 |
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| 31.2 |
0.314 |
0.301 |
0.308 |
0.183 |
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| 15.6 |
0.207 |
0.202 |
0.205 |
0.080 |
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| 7.8 |
0.153 |
0.159 |
0.156 |
0.031 |
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| 0 |
0.127 |
0.123 |
0.125 |
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货期
3-5 working days
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