产品描述
This Pig PDGF-AB ELISA Kit was designed for the quantitative measurement of Pig PDGF-AB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
缩写
PDGF-AB
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig PDGF-AB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 95 | | |
| Range % | 85-103 | | |
| 1:2 | Average % | 90 | | |
| Range % | 85-99 | | |
| 1:4 | Average % | 93 | | |
| Range % | 84-97 | | |
| 1:8 | Average % | 95 | | |
| Range % | 83-100 | | |
回收率
| The recovery of pig PDGF-AB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 95 | 83-101 | | |
| EDTA plasma (n=4) | 93 | 87-100 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | | 20 | 2.410 | 2.453 | 2.432 | 2.250 | | | | 10 | 1.573 | 1.565 | 1.569 | 1.387 | | | | 5 | 0.988 | 0.997 | 0.993 | 0.811 | | | | 2.5 | 0.609 | 0.621 | 0.615 | 0.433 | | | | 1.25 | 0.429 | 0.438 | 0.434 | 0.252 | | | | 0.625 | 0.347 | 0.335 | 0.341 | 0.159 | | | | 0.312 | 0.245 | 0.242 | 0.244 | 0.062 | | | | 0 | 0.180 | 0.183 | 0.182 | | | |
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货期
3-5 working days