产品描述
This Human IL26 ELISA Kit was designed for the quantitative measurement of Human IL26 protein in serum, urine, tissue homogenates, cell lysates, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.
别名
AK155 ELISA Kit; IL 26 ELISA Kit; IL-26 ELISA Kit; IL26 ELISA Kit; IL26_HUMAN ELISA Kit; Interleukin 26 ELISA Kit; Interleukin-26 ELISA Kit; Protein AK155 ELISA Kit
缩写
IL26
Uniprot No
Q9NPH9
灵敏度
15.6 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-26 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
96 |
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| Range % |
90-102 |
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| 1:2 |
Average % |
89 |
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| Range % |
84-95 |
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| 1:4 |
Average % |
100 |
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| Range % |
95-106 |
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| 1:8 |
Average % |
97 |
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| Range % |
92-104 |
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回收率
| The recovery of human IL-26 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
93-96 |
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| EDTA plasma (n=4) |
102 |
98-107 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 4000 |
2.749 |
2.798 |
2.774 |
2.616 |
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| 2000 |
2.315 |
2.364 |
2.340 |
2.182 |
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| 1000 |
1.843 |
1.889 |
1.866 |
1.708 |
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| 500 |
1.399 |
1.381 |
1.390 |
1.232 |
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| 250 |
0.899 |
0.888 |
0.894 |
0.736 |
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| 125 |
0.483 |
0.487 |
0.485 |
0.327 |
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| 62.5 |
0.316 |
0.329 |
0.323 |
0.165 |
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| 0 |
0.157 |
0.159 |
0.158 |
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货期
3-5 working days