产品描述

This Human KLKB1 ELISA Kit was designed for the quantitative measurement of Human KLKB1 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 9.38 mU/mL-600 mU/mL and the sensitivity is 2.34 mU/mL.

别名

Fletcher factor ELISA Kit; kallikrein B plasma ELISA Kit; Kallikrein B plasma (Fletcher factor) 1 ELISA Kit; Kallikrein B; plasma 1 ELISA Kit; Kallikrein B1 ELISA Kit; Kininogenin ELISA Kit; KLK3 ELISA Kit; KLKB1 ELISA Kit; KLKB1_HUMAN ELISA Kit; PKK ELISA Kit; PKKD ELISA Kit; Plasma kallikrein ELISA Kit; Plasma kallikrein B1 ELISA Kit; Plasma kallikrein light chain ELISA Kit; Plasma prekallikrein ELISA Kit; PPK ELISA Kit; Prekallikrein ELISA Kit

缩写

KLKB1

Uniprot No

P03952

灵敏度

2.34 mU/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human KLKB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:100		Average %		96
		Range %		89-100
1:200		Average %		90
		Range %		85-98
1:400		Average %		87
		Range %		83-93
1:800		Average %		92
		Range %		88-96

回收率

The recovery of human KLKB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		80-103
EDTA plasma (n=4)		96		86-106

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml OD1 OD2 Average Corrected 600 2.101 2.207 2.154 2.048 300 1.695 1.687 1.691 1.585 150 1.261 1.278 1.270 1.164 75 0.842 0.867 0.855 0.749 37.5 0.503 0.514 0.509 0.403 18.75 0.345 0.368 0.357 0.251 9.38 0.201 0.211 0.206 0.100 0 0.105 0.107 0.106

货期

3-5 working days