产品描述
This Sheep IL10 ELISA Kit was designed for the quantitative measurement of Sheep IL10 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 5 pg/mL-80 pg/mL and the sensitivity is 5 pg/mL.
别名
IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
缩写
IL10
Uniprot No
Q29408
灵敏度
5 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
87-98 |
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| 1:2 |
Average % |
98 |
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| Range % |
93-104 |
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| 1:4 |
Average % |
84 |
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| Range % |
82-88 |
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| 1:8 |
Average % |
90 |
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| Range % |
85-97 |
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回收率
| The recovery of sheep IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
91-99 |
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| EDTA plasma (n=4) |
92 |
87-97 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 80 |
0.202 |
0.196 |
0.199 |
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| 40 |
0.365 |
0.345 |
0.355 |
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| 20 |
0.623 |
0.641 |
0.632 |
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| 10 |
1.147 |
1.168 |
1.158 |
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| 5 |
2.344 |
2.189 |
2.267 |
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货期
3-5 working days