产品描述

This Human NOS2 ELISA Kit was designed for the quantitative measurement of Human NOS2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.9 IU/mL-60 IU/mL and the sensitivity is 0.225 IU/mL .

别名

HEP-NOS ELISA Kit; Hepatocyte NOS ELISA Kit; HEPNOS ELISA Kit; inducible ELISA Kit; Inducible nitric oxide synthase ELISA Kit; Inducible NO synthase ELISA Kit; Inducible NOS ELISA Kit; iNOS ELISA Kit; MAC NOS ELISA Kit; Macrophage NOS ELISA Kit; Nitric oxide synthase 2 inducible ELISA Kit; Nitric oxide synthase 2 inducible macrophage ELISA Kit; nitric oxide synthase 2A (inducible, hepatocytes) ELISA Kit; Nitric oxide synthase ELISA Kit; Nitric oxide synthase inducible ELISA Kit; nitric oxide synthase, macrophage ELISA Kit; NOS 2 ELISA Kit; NOS ELISA Kit; Nos II ELISA Kit; NOS type II ELISA Kit; nos2 ELISA Kit; NOS2_HUMAN ELISA Kit; NOS2A ELISA Kit; NOS2A, Inducible, Hepatocyte ELISA Kit; Peptidyl-cysteine S-nitrosylase NOS2 ELISA Kit

缩写

NOS2

Uniprot No

P35228

灵敏度

0.225 IU/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Neuroscience

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human iNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		93
		Range %		89-95
1:2		Average %		103
		Range %		99-105
1:4		Average %		94
		Range %		90-97
1:8		Average %		99
		Range %		95-101

回收率

The recovery of human iNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		92-97
EDTA plasma (n=4)		96		92-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

IU/ml OD1 OD2 Average Corrected 60 2.519 2.499 2.509 2.378 30 1.934 1.876 1.905 1.774 15 1.277 1.281 1.279 1.148 7.5 0.695 0.683 0.689 0.558 3.75 0.442 0.434 0.438 0.307 1.8 0.317 0.302 0.310 0.179 0.9 0.218 0.225 0.222 0.091 0 0.133 0.129 0.131

货期

3-5 working days