产品描述
This human LAMA5 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human LAMA5 in serum and plasma. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of LAMA5 bound in the initial step.
The LAMA5 gene codes for the laminin subunit α5, the most abundant laminin α subunit in the human brain. It forms a heterotrimer with subunit β1/β2 and γ1/γ3 and modulates neurodevelopmental processes, including epiblast polarization, neurite outgrowth, neuronal migration, synaptic stability, cell adhesion, differentiation, migration, and signaling transduction. LAMA5 plays a prominent role in the maintenance and function of the extracellular matrix (ECM) in mammalian tissues, which is crucial in developmental patterning, stem cell niches, cancer, and genetic diseases. It is necessary for normal embryonic development.
别名
KIAA0533 ELISA Kit; KIAA1907 ELISA Kit; LAMA5 ELISA Kit; LAMA5_HUMAN ELISA Kit; Laminin alpha 5 chain ELISA Kit; Laminin subunit alpha 5 ELISA Kit; Laminin subunit alpha-5 ELISA Kit; Laminin; alpha 5 ELISA Kit; Laminin-10 subunit alpha ELISA Kit; Laminin-11 subunit alpha ELISA Kit; Laminin-15 subunit alpha ELISA Kit; RP11-157P1.6 ELISA Kit
缩写
LAMA5
Uniprot No
O15230
灵敏度
3.12 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of Human LN-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 88 | |
| Range % | 84-94 | |
| 1:2 | Average % | 98 | |
| Range % | 92-102 | |
| 1:4 | Average % | 95 | |
| Range % | 90-100 | |
| 1:8 | Average % | 102 | |
| Range % | 96-106 | |
回收率
| The recovery of Human LN-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 88-98 | |
| EDTA plasma (n=4) | 86 | 82-92 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 800 | 2.262 | 2.368 | 2.315 | 2.216 | | | 400 | 1.676 | 1.570 | 1.623 | 1.524 | | | 200 | 0.987 | 0.960 | 0.974 | 0.875 | | | 100 | 0.558 | 0.547 | 0.553 | 0.454 | | | 50 | 0.312 | 0.324 | 0.318 | 0.219 | | | 25 | 0.243 | 0.256 | 0.250 | 0.151 | | | 12.5 | 0.172 | 0.174 | 0.173 | 0.074 | | | 0 | 0.098 | 0.099 | 0.099 | | |
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货期
3-5 working days
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