产品描述

This Human NEP ELISA Kit was designed for the quantitative measurement of Human NEP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

别名

Atriopeptidase ELISA Kit; CALLA ELISA Kit; CD10 ELISA Kit; CD10 antigen ELISA Kit; Common acute lymphocytic leukemia antigen ELISA Kit; DKFZp686O16152 ELISA Kit; EC 3.4.24.11 ELISA Kit; Enkephalinase ELISA Kit; EPN ELISA Kit; Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) ELISA Kit; Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) ELISA Kit; Membrane metallo endopeptidase ELISA Kit; Membrane metallo endopeptidase variant 1 ELISA Kit; Membrane metallo endopeptidase variant 2 ELISA Kit; Membrane metalloendopeptidase ELISA Kit; Membrane metalloendopeptidase neutral endopeptidase enkephalinase ELISA Kit; Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 ELISA Kit; Membrane metalloendopeptidase variant 1 ELISA Kit; Membrane metalloendopeptidase variant 2 ELISA Kit; MGC126681 ELISA Kit; MGC126707 ELISA Kit; MME ELISA Kit; NEP ELISA Kit; NEP_HUMAN ELISA Kit; Neprilysin ELISA Kit; neprilysin-390 ELISA Kit; neprilysin-411 ELISA Kit; Neutral endopeptidase 24.11 ELISA Kit; Neutral endopeptidase ELISA Kit; Neutral endopeptidase, membrane-associated ELISA Kit; SFE ELISA Kit; Skin fibroblast elastase ELISA Kit

缩写

NEP

Uniprot No

P08473

灵敏度

19.5 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Neuroscience

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human NEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		91
		Range %		86-95
1:2		Average %		97
		Range %		90-104
1:4		Average %		103
		Range %		97-109
1:8		Average %		89
		Range %		85-93

回收率

The recovery of human NEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		88-96
EDTA plasma (n=4)		97		90-104

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   5000 2.388 2.580 2.484 2.340   2500 1.836 1.947 1.892 1.748   1250 1.471 1.425 1.448 1.304   625 0.925 1.005 0.965 0.821   312.5 0.605 0.654 0.630 0.486   156 0.388 0.389 0.389 0.245   78 0.249 0.244 0.247 0.103   0 0.138 0.149 0.144

货期

3-5 working days