产品描述
KL(Klotho)是一种跨膜蛋白,最初在老鼠的基因研究中发现,对寿命具有显著的调节作用。KL主要分布在肾小管和脑部神经元中,是一种重要的生长因子和信号分子,参与调节细胞代谢、能量代谢、细胞凋亡等生物学过程。KL还与多种疾病的发生和发展相关,包括糖尿病、肾脏疾病、神经系统疾病等。
华美生物所提供的Human Klotho ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的KL,其灵敏度为0.039 ng/mL,检测范围为0.156 ng/mL-10 ng/mL。
别名
Kl ELISA Kit; KLOT_HUMAN ELISA Kit; Klotho peptide ELISA Kit
缩写
KL
Uniprot No
Q9UEF7
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Developmental Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human Klotho in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
86-92 |
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| 1:2 |
Average % |
98 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
96 |
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| Range % |
92-102 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-99 |
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回收率
| The recovery of human Klotho spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
89-98 |
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| EDTA plasma (n=4) |
97 |
90-102 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.491 |
2.452 |
2.472 |
2.350 |
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| 5 |
2.104 |
2.076 |
2.090 |
1.968 |
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| 2.5 |
1.504 |
1.562 |
1.533 |
1.411 |
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| 1.25 |
0.869 |
0.931 |
0.900 |
0.778 |
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| 0.625 |
0.494 |
0.507 |
0.501 |
0.379 |
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| 0.312 |
0.292 |
0.279 |
0.286 |
0.164 |
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| 0.156 |
0.185 |
0.179 |
0.182 |
0.060 |
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| 0 |
0.126 |
0.117 |
0.122 |
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货期
3-5 working days