产品描述

This Human NTF4 ELISA Kit was designed for the quantitative measurement of Human NTF4 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL .

别名

GLC10 ELISA Kit; GLC1O ELISA Kit; Neurotrophic factor 4 ELISA Kit; Neurotrophic factor 5 ELISA Kit; Neurotrophin 4 ELISA Kit; Neurotrophin 4/5 ELISA Kit; Neurotrophin 5 (neurotrophin 4/5) ELISA Kit; Neurotrophin 5 ELISA Kit; Neurotrophin-4 ELISA Kit; Neurotrophin-5 ELISA Kit; Neutrophic factor 4 ELISA Kit; Neutrophic factor 5 ELISA Kit; NT 4 ELISA Kit; NT 4/5 ELISA Kit; NT 5 ELISA Kit; NT-4 ELISA Kit; NT-5 ELISA Kit; NT4 ELISA Kit; NT4/5 ELISA Kit; NT4P ELISA Kit; NT5 ELISA Kit; NTF4 ELISA Kit; NTF4_HUMAN ELISA Kit; NTF5 ELISA Kit

缩写

NTF4

Uniprot No

P34130

灵敏度

0.156 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Neuroscience

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human NT-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		88-95
1:2		Average %		103
		Range %		99-106
1:4		Average %		93
		Range %		87-96
1:8		Average %		97
		Range %		93-100

回收率

The recovery of human NT-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		90-97
EDTA plasma (n=4)		96		92-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 40 2.160 2.145 2.153 2.013 20 1.676 1.740 1.708 1.568 10 1.208 1.188 1.198 1.058 5 0.862 0.826 0.844 0.704 2.5 0.602 0.616 0.609 0.469 1.25 0.442 0.434 0.438 0.298 0.625 0.296 0.278 0.287 0.147 0 0.142 0.138 0.140

货期

3-5 working days